No, he can’t be looking at each position if he’s changing 10 at a time. Your claim is utterly false.
So you are claiming because he changes 10 at a time he cannot estimate substitutability?
how is that matter? what about the experiments he does mention? how you explain that all these experiments show similar (high) result?
I meant what I wrote, Bill. You claimed he was looking at each position. That’s an objectively false claim. Put down the goalposts.
They are outliers.
Bad luck. Poor methodology. Why didn’t he mention the hundreds of catalytic antibodies already published at the time he wrote the paper, for example?
I will modify my claim that he is estimating substitutability at each position. Thanks for the correction.
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i dont know. you should ask him but if these other examples are correct i dont think its so relevant. on the other- can you explain to me why we never seen someone that take a bunch of random sequences to evolve small proteins like histon or globin or cytochrome? if your numbers are true for any tipical protein it should be very easy to do such experiment.
From no resistance to resistance, yes. Here you go:
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