That’s what he writes. Is it true, Lee? Is that how we should assess the minimal constraints for proper enzyme function?
Assuming that is what you want to find, I’d think, and I’d be curious if you have a different opinion(?), that what we should do is find the lowest concentration of antibiotic that measurably affects fitness, then find what the lowest enzymatic activity that is necessary to produce a measurable fitness advantage is. That means, I think, that we have to experiment with increasingly diluted concetrations of antibiotic and measure doubling times (usually by OD) of the bacteria in question, until we can no longer distinguis the effect of antibiotic on growth rate from just experimental noise. Once we’ve done that, we’ve found the lowest threshold where we can at least measurably show that an enzyme should have a fitness effect. Then we can proceed to measure the effects on doubling time with bacteria encoding enzymes of different activities (themselves established first by assays of enzyme kinetics).
I think that’s what we’d want to do if we truly were trying to establish “the minimal constraints for proper enzyme function.”
Are you going to engage your own brain and think about this, or are you just going to feed us this bs line about taking it up with the journal because you yourself aren’t smart enough to work this stuff out and all you ever do is blindly parrot what the clever academics on your side say to you?